Počet záznamů: 1
Phylogenetic and Virulence Analysis of Tick-Borne Encephalitis Virus Field Isolates From Switzerland
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SYSNO ASEP 0359444 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Phylogenetic and Virulence Analysis of Tick-Borne Encephalitis Virus Field Isolates From Switzerland Tvůrce(i) Gaumann, R. (CH)
Růžek, Daniel (BC-A) RID, ORCID
Muhlemann, K. (CH)
Strasser, M. (CH)
Beuret, C. M. (CH)Zdroj.dok. Journal of Medical Virology - ISSN 0146-6615
Roč. 83, č. 5 (2011), 853-863Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova molecular epidemiology ; envelope protein ; neuroinvasiveness/virulence ; European subtype ; Ixodes ricinus Vědní obor RIV EE - Mikrobiologie, virologie CEP GPP302/10/P438 GA ČR - Grantová agentura ČR LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000288464400016 DOI 10.1002/jmv.21993 Anotace Tick-borne encephalitis (TBE) is an endemic disease in Switzerland, with about 110-120 reported human cases each year. In this study, the complete envelope (E) protein sequences and phylogenetic classification of 72 TBE viruses found in Ixodes ricinus ticks sampled at 39 foci throughout Switzerland were analyzed. All isolates belonged to the European subtype and were highly. Sixty-four isolates were characterized in vitro with respect to their plaque phenotype. All of six virus isolates investigated in an in vivo mouse model were highly neurovirulent (100% mortality) but exhibited a relatively low level of neuroinvasiveness, with mouse survival rates ranging from 50% to 100%. Therefore, TBE viruses circulating in Switzerland belong to the European subtype and are closely related. In vitro and in vivo surrogates suggest a high proportion of isolates with a relatively low level of virulence, which is in agreement with a hypothesized high proportion of subclinical or mild TBE infections. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1