Počet záznamů: 1

MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

SYSNO ASEP	0359407
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process
Tvůrce(i)	Ammerman, M. L. (US) Hashimi, Hassan (BC-A)_{RID, ORCID} Novotná, Lucie (BC-A) Číčová, Zdeňka (BC-A) Mcevoy, S. M. (US) Lukeš, Julius (BC-A)_{RID, ORCID} Read, L. K. (US)
Zdroj.dok.	RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382 Roč. 17, č. 5 (2011), 865-877
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	RNA editing ; trypanosome ; MRB1 complex ; mitochondria ; kinetoplast
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA204/09/1667 GA ČR - Grantová agentura ČR
	2B06129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LC07032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000289617400009
DOI	10.1261/rna.2446311
Anotace	In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1