Počet záznamů: 1

Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones

SYSNO ASEP	0359306
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones
Tvůrce(i)	Frébortová, Jitka (UEB-Q)_{RID, ORCID} Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Frébort, Ivo (UEB-Q) Jorda, Radek (UEB-Q)_{ORCID, RID}
Celkový počet autorů	4
Zdroj.dok.	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 61, č. 3 (2010), s. 467-481
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Degradation ; cytokinins ; maize
Vědní obor RIV	EF - Botanika
CEP	GA522/05/0448 GA ČR - Grantová agentura ČR
	GA301/08/1649 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000273911800009
DOI	10.1111/j.1365-313X.2009.04071.x
Anotace	Hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one (DIMBOA) was isolated from maize phloem sap as a compound enhancing the degradation of isopentenyl adenine by maize cytokinin dehydrogenase (CKX), after oxidative conversion by either laccase or peroxidase. Laccase and peroxidase catalyze oxidative cleavage of DIMBOA to 4-nitrosoresorcinol-1-monomethyl ether (coniferron), which serves as a weak electron acceptor of CKX. The oxidation of DIMBOA and coniferron generates transitional free radicals that are used by CKX as effective electron acceptors. The function of free radicals in the CKX-catalyzed reaction was also verified with a stable free radical of 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Application of exogenous cytokinin to maize seedlings resulted in an enhanced benzoxazinoid content in maize phloem sap. The results indicate a new function for DIMBOA in the metabolism of the cytokinin group of plant hormones.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1