Počet záznamů: 1
PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca
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SYSNO ASEP 0359234 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca Tvůrce(i) Lajbner, Zdeněk (UZFG-Y) RID
Kotlík, Petr (UZFG-Y) RID, ORCIDZdroj.dok. Molecular Ecology Resources - ISSN 1755-098X
Roč. 11, č. 2 (2011), s. 374-377Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova EPIC ; exon-primed intron-crossing marker ; mtDNA Vědní obor RIV EG - Zoologie CEP LC06073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000287200200019 DOI 10.1111/j.1755-0998.2010.02914.x Anotace The tench Tinca tinca is a valued table fish which is being transplanted between watersheds without concern for genetic similarity to wild populations, and efficient phylogeographic markers were therefore urgently needed to rapidly distinguish genetically distinct geographic populations and to assess their contribution to the hatchery breeds and to the stocked wild populations. The new method developed in this study relies on PCR-RFLP assays of two independent nuclear-encoded markers and of one mitochondrial DNA marker, and allows the rapid identification of the Western and Eastern phylogroup and also of three geographical mtDNA clades within the Eastern phylogroup. The method will be useful for monitoring the introduction and human-mediated spread of the phylogroups in wild populations, for characterisation of cultured strains and in breeding experiments. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
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