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Monte Carlo simulation of fluorescence correlation spectroscopy data
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SYSNO ASEP 0358508 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Monte Carlo simulation of fluorescence correlation spectroscopy data Tvůrce(i) Košovan, P. (CZ)
Uhlík, F. (CZ)
Kuldová, J. (CZ)
Štěpánek, M. (CZ)
Limpouchová, Z. (CZ)
Procházka, K. (CZ)
Benda, Aleš (UFCH-W) RID, ORCID
Humpolíčková, Jana (UFCH-W) RID
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Collection of Czechoslovak Chemical Communications. - : Ústav organické chemie a biochemie AV ČR, v. v. i. - ISSN 0010-0765
Roč. 76, č. 3 (2011), s. 207-222Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Monte Carlo Study ; fluorescence ; spectroscopy Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP IAA400400621 GA AV ČR - Akademie věd CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000288340200005 DOI 10.1135/cccc2009526 Anotace We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2012
Počet záznamů: 1