Počet záznamů: 1
Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families
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SYSNO ASEP 0358401 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families Tvůrce(i) Novotná, Jana (BC-A)
Havelka, Jan (BC-A) RID
Starý, Petr (BC-A) RID
Koutecký, P. (CZ)
Vítková, Magda (BC-A) RIDCelkový počet autorů 5 Zdroj.dok. Genetica - ISSN 0016-6707
Roč. 139, č. 3 (2011), s. 281-289Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Diuraphis noxia ; karyotype ; genome size Vědní obor RIV EB - Genetika a molekulární biologie CEP GA522/09/1940 GA ČR - Grantová agentura ČR CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000288514100001 DOI 10.1007/s10709-011-9546-4 Anotace We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg, whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2012
Počet záznamů: 1