Počet záznamů: 1

Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures

SYSNO ASEP	0357035
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures
Tvůrce(i)	Vondráková, Zuzana (UEB-Q)_{RID, ORCID} Cvikrová, Milena (UEB-Q)_{RID, ORCID} Eliášová, Kateřina (UEB-Q)_{RID, ORCID} Martincová, Olga (UEB-Q) Vágner, Martin (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Tree Physiology - ISSN 0829-318X Roč. 30, č. 10 (2010), s. 1335-1348
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	cryopreservation ; Norway spruce (Picea abies (L ) Karst) ; somatic embryogenesis
Vědní obor RIV	EA - Morfologické obory a cytologie
CEP	OC 158 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	OC08013 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000282818100009
DOI	10.1093/treephys/tpq074
Anotace	The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1