Počet záznamů: 1

In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

SYSNO ASEP	0355300
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies
Tvůrce(i)	Novotný, Ivan (UMG-J) Blažíková, Michaela (UEM-P) Staněk, David (UMG-J)_RID Heřman, P. (CZ) Malínský, Jan (UEM-P)_{RID, ORCID}
Zdroj.dok.	Molecular Biology of the Cell - ISSN 1059-1524 Roč. 22, č. 4 (2011), s. 513-523
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	small nuclear ribonucleoprotein ; body components ; dynamics
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA204/07/0133 GA ČR - Grantová agentura ČR
	KAN200520801 GA AV ČR - Akademie věd
CEZ	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
UT WOS	000287305700008
DOI	10.1091/mbc.E10-07-0560
Anotace	The U4/U6.U5 tri-snRNP is an essential pre-mRNA splicing factor, which is assembled in a step-wise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs) but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching (FRAP). A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. The rates of di-snRNP and tri-snRNP assemblies are similar and ca 230 tri-snRNPs are assembled in one CB per minute. Tri-snRNP assembly is about ten-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems.
Pracoviště	Ústav molekulární genetiky
Kontakt	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Rok sběru	2011

Počet záznamů: 1