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Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA
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SYSNO ASEP 0354847 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA Tvůrce(i) Bouřa, E. (CZ)
Řežábková, L. (CZ)
Brynda, Jiří (UMG-J) RID
Obšilová, Veronika (FGU-C) RID, ORCID, SAI
Obšil, T. (CZ)Zdroj.dok. Acta Crystallographica Section D-Biological Crystallography. - : WILEY-BLACKWELL - ISSN 0907-4449
Roč. 66, č. 12 (2010), s. 1351-1357Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. DK - Dánsko Klíč. slova transcription factor AFX ; posttranslational modification ; specificity Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA501110801 GA AV ČR - Akademie věd LC554 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000284881300012 DOI 10.1107/S0907444910042228 Anotace Here, the 1.9 A resolution crystal structure of the DNA-binding domain of human FOXO4 (FOXO4-DBD) bound to a 13 bp DNA duplex containing a FOXO consensus binding sequence is reported. The structure shows a similar recognition of the core sequence as has been shown for two other FOXO proteins. Helix H3 is docked into the major groove and provides all of the base-specific contacts, while the N-terminus and wing W1 make additional contacts with the phosphate groups of DNA. In contrast to other FOXO-DBD-DNA structures, the loop between helices H2 and H3 has a different conformation and participates in DNA binding. In addition, the structure of the FOXO4-DBD-DNA complex suggests that both direct water-DNA base contacts and the unique water-DNA base contacts and the unique water-network interactions contribute to FOXO-DBD binding to the DNA in a sequence-specific manner. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
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