Počet záznamů: 1
The Indispensable N-Terminal Half of elF3j/HCR1 Cooperates with its Structurally Conserved Binding Partner eIF3b/PRT1-RRM and with eIF1A in Stringent AUG Selection
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SYSNO ASEP 0354155 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Indispensable N-Terminal Half of elF3j/HCR1 Cooperates with its Structurally Conserved Binding Partner eIF3b/PRT1-RRM and with eIF1A in Stringent AUG Selection Tvůrce(i) ElAntak, L. (GB)
Wagner, Susan (MBU-M) RID, ORCID
Herrmannová, Anna (MBU-M) RID, ORCID
Karásková, Martina (MBU-M)
Rutkai, Edit (MBU-M)
Lukavsky, P. J. (GB)
Valášek, Leoš (MBU-M) RIDZdroj.dok. Journal of Molecular Biology. - : Elsevier - ISSN 0022-2836
Roč. 396, č. 4 (2010), s. 1097-1116Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova translation initiation ; AUG recognition ; eIF3 Vědní obor RIV CE - Biochemie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000275385600021 DOI 10.1016/j.jmb.2009.12.047 Anotace Despite the recent progress in our understanding of the numerous functions of individual subunits of eukaryotic translation initiation factor 3 (eIF3), there is still only little known on the molecular level. Using NMR spectroscopy, we determined the first solution structure of an interaction between eIF3 subunits. We revealed that a conserved tryptophan residue in the human eIF3j N-terminal acidic domain (NTA) is held in the helix α1 – loop L5 hydrophobic pocket of the human eIF3b-RRM. Mutating the corresponding “pocket” residues in its yeast orthologue reduces cellular growth rate, eliminates eIF3j/HCR1 association with eIF3b/PRT1 in vitro and in vivo, affects 40S-occupancy of eIF3, and produces a leaky scanning defect indicative of a deregulation of the AUG selection process. Unexpectedly, we found that the N-terminal half (NTD) of eIF3j/HCR1 containing the NTA motif is indispensable and sufficient for wild-type growth of yeast cells Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1