Počet záznamů: 1
Attenuation of vaccinia virus by the expression of human Flt3 ligand
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SYSNO ASEP 0353377 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Attenuation of vaccinia virus by the expression of human Flt3 ligand Tvůrce(i) Žurková, K. (CZ)
Hainz, P. (CZ)
Kryštofová, J. (CZ)
Kutinová, L. (CZ)
Šanda, Miloslav (UOCHB-X)
Němečková, Š. (CZ)Celkový počet autorů 6 Zdroj.dok. Virology Journal
Roč. 7, č. 1 (2010), 109/1-109/15Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova vaccinia virus ; antibodies ; virulence Vědní obor RIV CE - Biochemie CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000279552600004 DOI 10.1186/1743-422X-7-109 Anotace The expression of Flt3 ligand (FL) by recombinant vaccinia virus (rVACV) highly influenced properties of the virus in dependence on the level of expression. High production of FL driven by the strong synthetic promoter decreased the growth of rVACV in macrophage cell line J774.G8 in vitro as well as its multiplication in vivo when inoculated in mice. The inhibition of replication in vivo was mirrored in low levels of antibodies against vaccinia virus (anti-VACV) which nearly approached to the negative serum level in non-infected mice. Strong FL expression changed not only the host range of the recombinant but also the basic protein contents of virions. The major proteins - H3L and D8L - which are responsible for the virus binding to the cells, and 28 K protein that serves as a virulence factor, were changed in the membrane portion of P13-E/L-FL viral particles. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2011
Počet záznamů: 1