Počet záznamů: 1
Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth
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SYSNO ASEP 0352642 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Tvůrce(i) Kameník, Zdeněk (MBU-M) RID, ORCID
Hadacek, F. (AT)
Marečková, M. (CZ)
Ulanová, Dana (MBU-M)
Kopecký, J. (CZ)
Chobot, V. (AT)
Plháčková, Kamila (MBU-M) RID
Olšovská, Jana (MBU-M)Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1217, č. 51 (2010), s. 8016-8025Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova UHPLC ; high-throughput fingerprinting ; secondary metabolites Vědní obor RIV CB - Analytická chemie, separace CEP 2B08064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000285429700012 DOI 10.1016/j.chroma.2010.08.031 Anotace A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1