Počet záznamů: 1
Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato
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SYSNO ASEP 0352451 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato Tvůrce(i) Diermann, N. (DE)
Matoušek, Jaroslav (BC-A) RID, ORCID
Junge, M. (DE)
Riesner, D. (DE)
Steger, G. (DE)Zdroj.dok. Biological Chemistry. - : Walter de Gruyter - ISSN 1431-6730
Roč. 391, č. 12 (2010), s. 1379-1390Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova microRNAs ; transcription factor ; viroid pathogenicity Vědní obor RIV EB - Genetika a molekulární biologie CEP GA521/08/0740 GA ČR - Grantová agentura ČR 1QS500510558 GA AV ČR - Akademie věd CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000284586700003 DOI 10.1515/BC.2010.148 Anotace To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs. Though they also cause the production of viroid-specific small RNAs (vsRNA), viroids do escape the cytoplasmic silencing mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement of vsRNAs in symptom production. We analyzed by deep sequencing the spectrum of vsRNAs induced by a severe PSTVd strain, AS1, upon infection of tomato plants cv Rutgers. Indeed, vsRNAs found with highest frequency mapped to the pathogenicity-modulating domain of PSTVd, supporting an involvement of vsRNAs in symptom production. Furthermore, in PSTVd AS1-infected plants the accumulation of some endogenous miRNAs, which are involved in leaf development via regulation of transcription factors, is suppressed. The latter finding supports the hypothesis that a miRNA-dependent (mis)regulation of transcription factors causes the viroid symptoms. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1