Počet záznamů: 1
Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae
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SYSNO ASEP 0352047 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae Tvůrce(i) Seetharaman, A. (CA)
Cumbo, P. (CA)
Bojanala, Nagagireesh (BC-A)
Gupta, B. P. (CA)Zdroj.dok. Developmental Biology. - : Elsevier - ISSN 0012-1606
Roč. 346, č. 1 (2010), s. 128-139Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Nematode ; C. elegans ; C. briggsae ; Vulval development ; Signal transduction ; Wnt signaling Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000281930900011 DOI https://doi.org/10.1016/j.ydbio.2010.07.003 Anotace To understand the function and evolution of Wnt signaling in Caenorhabditis nematodes we focused on C. briggsae, a species that is substantially divergent from C. elegans in terms of the evolutionary time scale yet shares almost identical morphology. We isolated mutants in C briggsae that display multiple pseudo-vulvae resulting from ectopic VPC induction. We cloned one of these loci and found that it encodes an Axin homolog, Cbr-PRY-1. Our genetic studies revealed that Cbr-pry-1 functions upstream of the canonical Wnt pathway components Cbr-bar-1 (beta-catenin) and Cbr-pop-1(tcf/lef) as well as the Hox target Cbr-lin-39 (Dfd/Scr). We further characterized the pry-1 vulval phenotype in C briggsae and C elegans using 8 cell fate markers, cell ablation, and genetic interaction approaches. Our results show that ectopically induced VPCs in pry-1 mutants adopt 2 degrees fates independently of the gonad-derived inductive and LIN-12/Notch-mediated lateral signaling pathways. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1