Počet záznamů: 1
High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula
- 1.
SYSNO ASEP 0351441 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula Tvůrce(i) Stodůlková, Eva (MBU-M) ORCID
Man, Petr (MBU-M) RID, ORCID
Kolařík, Miroslav (MBU-M) RID, ORCID
Flieger, Miroslav (MBU-M) ORCIDZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1217, č. 40 (2010), s. 6296-6302Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova High-performance liquid chromatography ; Mass spectrometry ; Anthraquinones Vědní obor RIV EE - Mikrobiologie, virologie CEP 2B08064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KAN200200651 GA AV ČR - Akademie věd CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000282725800020 DOI 10.1016/j.chroma.2010.08.009 Anotace Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1