Počet záznamů: 1
The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
- 1.
SYSNO ASEP 0351126 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells Tvůrce(i) Huranová, Martina (UMG-J) ORCID
Ivani, Ivan (UMG-J)
Benda, Aleš (UFCH-W) RID, ORCID
Poser, I. (DE)
Brody, Y. (IL)
Hof, Martin (UFCH-W) RID, ORCID
Shav-Tal, Y. (IL)
Neugebauer, K.M. (DE)
Staněk, David (UMG-J) RIDZdroj.dok. Journal of Cell Biology. - : Rockefeller University Press - ISSN 0021-9525
Roč. 191, č. 1 (2010), s. 75-86Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova FRAP ; RNA splicing ; spliceosome Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/07/0133 GA ČR - Grantová agentura ČR GAP305/10/0424 GA ČR - Grantová agentura ČR KAN200520801 GA AV ČR - Akademie věd LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000282648500009 DOI 10.1083/jcb.201004030 Anotace Precursor messenger RNA (pre-mRNA) splicing is catalyzed by the spliceosome, a large ribonucleoprotein (RNP) complex composed of five small nuclear RNP particles (snRNPs) and additional proteins. Using live cell imaging of GFP-tagged snRNP components expressed at endogenous levels, we examined how the spliceosome assembles in vivo. A comprehensive analysis of snRNP dynamics in the cell nucleus enabled us to determine snRNP diffusion throughout the nucleoplasm as well as the interaction rates of individual snRNPs with pre-mRNA. Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15-30 s, indicating that splicing is accomplished within this time period. Additionally, binding of U1 and U4/U6 snRNPs with pre-mRNA occurred within seconds, indicating that the interaction of individual snRNPs with pre-mRNA is distinct. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
Počet záznamů: 1