Počet záznamů: 1  

The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells

  1. 1.
    SYSNO ASEP0351126
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevThe differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
    Tvůrce(i) Huranová, Martina (UMG-J)
    Ivani, Ivan (UMG-J)
    Benda, Aleš (UFCH-W)
    Poser, I. (DE)
    Brody, Y. (IL)
    Hof, Martin (UFCH-W) RID
    Shav-Tal, Y. (IL)
    Neugebauer, K.M. (DE)
    Staněk, David (UMG-J) RID
    Zdroj.dok.Journal of Cell Biology - ISSN 0021-9525
    Roč. 191, č. 1 (2010), s. 75-86
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaFRAP ; RNA splicing ; spliceosome
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPGA204/07/0133 GA ČR - Grantová agentura ČR
    GAP305/10/0424 GA ČR - Grantová agentura ČR
    KAN200520801 GA AV ČR - Akademie věd
    LC06063 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    CEZAV0Z50520514 - UMG-J (2005-2011)
    AV0Z40400503 - UFCH-W (2005-2011)
    UT WOS000282648500009
    DOI10.1083/jcb.201004030
    AnotacePrecursor messenger RNA (pre-mRNA) splicing is catalyzed by the spliceosome, a large ribonucleoprotein (RNP) complex composed of five small nuclear RNP particles (snRNPs) and additional proteins. Using live cell imaging of GFP-tagged snRNP components expressed at endogenous levels, we examined how the spliceosome assembles in vivo. A comprehensive analysis of snRNP dynamics in the cell nucleus enabled us to determine snRNP diffusion throughout the nucleoplasm as well as the interaction rates of individual snRNPs with pre-mRNA. Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15-30 s, indicating that splicing is accomplished within this time period. Additionally, binding of U1 and U4/U6 snRNPs with pre-mRNA occurred within seconds, indicating that the interaction of individual snRNPs with pre-mRNA is distinct.
    PracovištěÚstav molekulární genetiky
    KontaktGabriela Marešová, maresova@img.cas.cz, Tel.: 241 063 217
    Rok sběru2011