Počet záznamů: 1

Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants

  1. 1.
    SYSNO ASEP0350379
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    NázevCharacterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants
    Tvůrce(i)Dwivedi, S. (US) - Vaňková, Radomíra (UEB-Q) - Motyka, Václav (UEB-Q) - Herrera, C. (US) - Žižková, Eva (UEB-Q) - Auer, C. (US)
    Rozsah stran231 - 242
    Zdroj.dok.Plant Growth Regulation - ISSN 0167-6903
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaCytokinin metabolism ; N-Glucosyltransferase ; GRAM domain
    Vědní obor RIVED - Fyziologie
    CEPIAA600380701 GA AV ČR - Akademie věd
    LC06034 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    ME 505 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    UTISI000278411500003
    DOI10.1007/s10725-010-9467-8
    AnotaceCytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2011