Počet záznamů: 1
Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells
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SYSNO ASEP 0350113 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells Tvůrce(i) Kunová, M. (CZ)
Matulka, K. (CZ)
Eiselleová, L. (CZ)
Trčková, P. (CZ)
Hampl, Aleš (UEM-P)
Dvořák, Petr (UEM-P)Zdroj.dok. Reproductive BioMedicine Online - ISSN 1472-6483
Roč. 21, - (2010), s. 676-686Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova animal protein-free culture ; high-density culture ; human embryonic stem cells Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000284797000016 DOI 10.1016/j.rbmo.2010.06.027 Anotace For human embryonic stem cells (hESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of hESC. Even under the most stringent feeder-free conditions without conditioned medium, hESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2011
Počet záznamů: 1