Počet záznamů: 1
Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting
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SYSNO ASEP 0349793 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting Tvůrce(i) Ascher, J. (IT)
Ceccherini, M.T. (IT)
Chroňáková, Alica (BC-A) RID, ORCID
Jirout, Jiří (BC-A) RID
Borgogni, F. (IT)
Elhottová, Dana (BC-A) RID, ORCID
Šimek, Miloslav (BC-A) RID, ORCID
Pietramellara, G. (IT)Zdroj.dok. World Journal of Microbiology and Biotechnology. - : Springer - ISSN 0959-3993
Roč. 26, č. 9 (2010), s. 1721-1726Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova cattle impact ; DGGE ; DGGE interfering factors Vědní obor RIV EH - Ekologie - společenstva CEP LC06066 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA600660605 GA AV ČR - Akademie věd CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000280642800021 DOI 10.1007/s11274-010-0349-z Anotace We compared two denaturing gradient gel electrophoresis (DGGE) systems-DCode (Biorad, Hercules, CA, USA) and PhorU (Ingeny, Leiden, NL), performing community level 16S and 18S rRNA gene fragment-PCR-DGGE with total DNA extracted from upland pasture soil used for outdoor cattle husbandry. The methodological evaluation of the DGGE apparatus as parameter influencing DGGE fingerprinting, based on cluster analysis of soil bacterial and fungal community fingerprints, was made in terms of the resulting information about microbial community structures and their response to different degrees of cattle impact. Although the comparative DGGE analysis with different DGGE systems provided similar clustering of microbial community structures in correlation with the degree of cattle impact, our results suggest the DGGE system to be a factor influencing DGGE analysis. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1