Počet záznamů: 1
High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity
- 1.
SYSNO ASEP 0349651 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity Tvůrce(i) Närvä, E. (FI)
Autio, R. (FI)
Rahkonen, N. (FI)
Kong, L. (FI)
Harrison, N. (GB)
Kitsberg, D. (IL)
Borghese, L. (DE)
Itskovitz-Eldor, J. (IL)
Rasool, O. (FI)
Dvořák, Petr (UEM-P)
Hovatta, O. (SE)
Otonkoski, T. (FI)
Tuuri, T. (FI)
Cui, W. (GB)
Brüstle, O. (GB)
Baker, D. (GB)
Maltby, E. (GB)
Moore, H. D. (GB)
Benvenisty, N. (IL)
Andrews, P.W. (GB)
Yli-Harja, O. (FI)
Lehesmaa, R. (FI)Zdroj.dok. Nature Biotechnology. - : Nature Publishing Group - ISSN 1087-0156
Roč. 28, č. 4 (2010), s. 371-U103Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DNA ; stem cell ; cancer Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50390512 - UEM-P (2005-2011) UT WOS 000276462400026 DOI 10.1038/nbt.1615 Anotace Prolonged culture of human embryonic stem cells (hESCs) can lead to adaptation and the acquisition of chromosomal abnormalities, underscoring the need for rigorous genetic analysis of these cells. Here we report the highest-resolution study of hESCs to date using an Affymetrix SNP 6.0 array containing 906,600 probes for single nucleotide polymorphisms (SNPs) and 946,000 probes for copy number variations (CNVs). Analysis of 17 different hESC lines maintained in different laboratories identified 843 CNVs of 50 kb-3 Mb in size. We identified, on average, 24% of the loss of heterozygosity (LOH) sites and 66% of the CNVs changed in culture between early and late passages of the same lines. Thirty percent of the genes detected within CNV sites had altered expression compared to samples with normal copy number states, of which >44% were functionally linked to cancer. Furthermore, LOH of the q arm of chromosome 16, which has not been observed previously in hESCs, was detected. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2011
Počet záznamů: 1