Počet záznamů: 1
Membrane Active Peptides. Methods and Results on Structure and Function
- 1.
SYSNO ASEP 0348380 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Peptide-Membrane Interactions Studied by Ellipsometry, Laser Scanning Microscopy, and Z-Scan Fluorescence Correlation Spectroscopy Tvůrce(i) Miszta, Adam (UFCH-W)
Macháň, Radek (UFCH-W)
Hermens, W. Th. (NL)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Membrane Active Peptides. Methods and Results on Structure and Function. - La Jolla : International University Line, 2010 / Castanho M. A. R. B. - ISBN 978-0-9720774-5-3 Rozsah stran s. 219-245 Poč.str. 27 s. Poč.str.knihy 635 Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ellipsometry ; laser scanning microscopy ; fluorescence microscopy Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40400503 - UFCH-W (2005-2011) DOI 10.3890/978-0-9720774-5-3 Anotace The following chapter presents two optical experimental techniques: confocal fluorescence microscopy (CFM) and ellipsometry, and focuses on their applications in studying interactions of antimicrobial peptides with supported phospholipid bilayers (SPBs). The principal properties of SPBs are mentioned together with their use as geometrically well-defined models of biological membranes. The basics of each of techniques are explained and their possibilities and limitations are discussed. Images with diffraction-limited resolution are obtained by CFM showing the spatial distribution of fluorescent molecules in the sample. Selective labeling offers the possibility to visualize location of different molecular species. Furthermore, the signal from each point of the sample can be analyzed separately; the measurement of molecular mobility in individual points of the sample by fluorescence correlation spectroscopy is addressed in more detail. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2011
Počet záznamů: 1