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Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy
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SYSNO ASEP 0347391 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy Tvůrce(i) Pšenička, M. (CZ)
Tesařová, Martina (BC-A) ORCID
Těšitel, J. (CZ)
Nebesářová, Jana (BC-A) RID, ORCIDZdroj.dok. Micron. - : Elsevier - ISSN 0968-4328
Roč. 41, č. 5 (2010), s. 455-460Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Scanning electron microscopy ; Size determination ; Sterlet spermatozoa ; Sterlet spermatozoa Vědní obor RIV EB - Genetika a molekulární biologie CEP KAN200520704 GA AV ČR - Akademie věd CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000278790400010 DOI https://doi.org/10.1016/j.micron.2010.02.004 Anotace In this study three types of scanning electron microscopes were used for the size determination of spermatozoa of sterlet Acipenser ruthenus - high vacuum scanning electron microscope (SEM, JEOL 6300), environmental scanning electron microscope (ESEM, Quanta 200 FEG), field emission scanning electron microscope (FESEM, JEOL 7401F) with cryoattachment Alto 2500 (Gatan) and transmission electron microscope (TEM, JEOL 1010). The use of particular microscopes was tied with different specimen preparation techniques. The aim of this study was to evaluate to what degree the type of used electron microscope can influence the size of different parts of spermatozoa. Results confirmed that the classical chemical procedure of specimen preparation for SEM including critical point drying method led to a significant contraction of all measured values, which could deviate up to 30% in comparison with values measured on the fresh chemically untreated specimen in ESEM. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
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