Počet záznamů: 1

Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy

SYSNO ASEP	0347391
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy
Tvůrce(i)	Pšenička, M. (CZ) Tesařová, Martina (BC-A)_ORCID Těšitel, J. (CZ) Nebesářová, Jana (BC-A)_{RID, ORCID}
Zdroj.dok.	Micron. - : Elsevier - ISSN 0968-4328 Roč. 41, č. 5 (2010), s. 455-460
Poč.str.	6 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Scanning electron microscopy ; Size determination ; Sterlet spermatozoa ; Sterlet spermatozoa
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	KAN200520704 GA AV ČR - Akademie věd
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000278790400010
DOI	10.1016/j.micron.2010.02.004
Anotace	In this study three types of scanning electron microscopes were used for the size determination of spermatozoa of sterlet Acipenser ruthenus - high vacuum scanning electron microscope (SEM, JEOL 6300), environmental scanning electron microscope (ESEM, Quanta 200 FEG), field emission scanning electron microscope (FESEM, JEOL 7401F) with cryoattachment Alto 2500 (Gatan) and transmission electron microscope (TEM, JEOL 1010). The use of particular microscopes was tied with different specimen preparation techniques. The aim of this study was to evaluate to what degree the type of used electron microscope can influence the size of different parts of spermatozoa. Results confirmed that the classical chemical procedure of specimen preparation for SEM including critical point drying method led to a significant contraction of all measured values, which could deviate up to 30% in comparison with values measured on the fresh chemically untreated specimen in ESEM.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2011

Počet záznamů: 1