Počet záznamů: 1
Extracellular complex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration
- 1.
SYSNO ASEP 0347007 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extracellular complex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration Tvůrce(i) Tishchenko, Galina (UMCH-V) RID
Koppová, Ingrid (UZFG-Y) RID
Šimůnek, Jiří (UZFG-Y) RID
Dohnálek, Jan (UMCH-V) RIDZdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 4 (2010), s. 386-389Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova chitinolytic enzymes ; Clostridium paraputrificum ; membrane diafiltration Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa CEP GA525/08/0803 GA ČR - Grantová agentura ČR GA310/09/1407 GA ČR - Grantová agentura ČR CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000281277900020 DOI 10.1007/s12223-010-0065-y Anotace Membrane diafiltration was used for separation of the extracellular complex of chitinolytic enzymes of C. paraputrificum J4 free from contaminants with molar mass higher than 100 kDa and lower than 30 kDa. The enzyme complex containing β-N-acetylglucosaminidase (NAGase) and six endochitinases was concentrated on a membrane with cut-off 30 kDa. In this retentate, the NAGase/endochitinase specific activity was 13.5/6.5-times higher than in the initial culture filtrate. NAGase (38 kDa) was less active and stable at pH lower than 4 and higher than 8 but it was more temperature-stable than endochitinases, especially at 40-60 °C. In contrast to endochitinases, the pH optimum of NAGase activity was shifted by ca. 0.7 pH units to the alkaline region. The knowledge of composition of chitinolytic enzymes, their pH and temperature stability is useful for optimization of the separation process. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2011
Počet záznamů: 1