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Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices
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SYSNO ASEP 0346926 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices Tvůrce(i) Coll, A. (ES)
Nadal, A. (ES)
Collado, R. (ES)
Capellades, G. (ES)
Kubista, Mikael (BTO-N) RID
Messeguer, J. (ES)
Pla, M. (ES)Zdroj.dok. Plant Molecular Biology. - : Springer - ISSN 0167-4412
Roč. 73, č. 3 (2010), s. 349-362Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova GMO (Genetically Modified Organism) ; MON810 ; Maize ; Transcriptome Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000276911800009 DOI 10.1007/s11103-010-9624-5 Anotace Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences between a given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810 had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2011
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