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Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas
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SYSNO ASEP 0346752 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas Tvůrce(i) Plachý, Jiří (UMG-J) RID
Kotáb, Jan (UMG-J)
Divina, Petr (UMG-J)
Reinišová, Markéta (UMG-J)
Šenigl, Filip (UMG-J) RID
Hejnar, Jiří (UMG-J) RIDZdroj.dok. Journal of Virology - ISSN 0022-538X
Roč. 84, č. 9 (2010), s. 4204-4211Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova avian sarcoma virus ; retrovirus integration ; position effects Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/07/1030 GA ČR - Grantová agentura ČR GP301/09/P667 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000276358000009 DOI 10.1128/JVI.02511-09 Anotace Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
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