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First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic

SYSNO ASEP	0345901
Druh ASEP	O - Ostatní výsledky
Zařazení RIV	O - Ostatní
Název	First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic
Tvůrce(i)	Přibylová, Jaroslava (BC-A)_{RID, ORCID} Špak, Josef (BC-A)_{RID, ORCID} Kubelková, Darina (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Celkový počet autorů	4
Zdroj.dok.	Plant Disease. - : American Phytopathological Society - ISSN 0191-2917 Roč. 94, č. 8 (2010), s. 1071-1071
Poč.str.	1 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Virus ; small fruits ; pathogen detection
Vědní obor RIV	EE - Mikrobiologie, virologie
CEP	OC09022 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000280058900045
DOI	10.1094/PDIS-94-8-1071B
Anotace	A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2013

Počet záznamů: 1