Počet záznamů: 1

Crystal structure and functional characterization of an immunomodulatory salivary cystatin from the soft tick Ornithodoros moubata

SYSNO ASEP	0345741
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Crystal structure and functional characterization of an immunomodulatory salivary cystatin from the soft tick Ornithodoros moubata
Tvůrce(i)	Salát, Jiří (BC-A)_{RID, ORCID} Paesen, G.C. (GB) Řezáčová, Pavlína (UOCHB-X)_{RID, ORCID} Kotsyfakis, Michalis (BC-A)_{RID, ORCID} Kovářová, Zuzana (UOCHB-X)_{RID, ORCID} Šanda, Miloslav (UOCHB-X) Majtán, J. (GB) Grunclová, Lenka (BC-A) Horká, Helena (BC-A)_RID Andersen, J. F. (US) Brynda, Jiří (UMG-J)_RID Horn, Martin (UOCHB-X)_{RID, ORCID} Nunn, M. A. (GB) Kopáček, Petr (BC-A)_{RID, ORCID} Kopecký, Jan (BC-A)_RID Mareš, Michael (UOCHB-X)_{RID, ORCID}
Zdroj.dok.	Biochemical Journal. - : Portland Press - ISSN 0264-6021 Roč. 429, č. 1 (2010), s. 103-112
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	cathepsin ; cystatin ; Ornithodoros moubata ; parasite ; peptidase inhibitor
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	KJB500960702 GA AV ČR - Akademie věd
	IAA600960811 GA AV ČR - Akademie věd
	GAP207/10/2183 GA ČR - Grantová agentura ČR
	LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000279418500011
DOI	10.1042/BJ20100280
Anotace	Using proteomic analysis, the cystatin OmC2 was demonstrated in the saliva of the soft tick Ornithodoros moubata. A structural, biochemical and biological characterization of this peptidase inhibitor was undertaken in the present study. Recombinant OmC2 was screened against a panel of physiologically relevant peptidases and was found to be an effective broad-specificity inhibitor of cysteine cathepsins, including endopeptidases (cathepsins L and S) and exopeptidases (cathepsins B, C and H). The crystal structure of OmC2 was determined at a resolution of 2.45 angstrom (1 angstrom = 0.1 nm) and was used to describe the structure inhibitory activity relationship. The biological impact of OmC2 was demonstrated both in vitro and in vivo. OmC2 affected the function of antigen-presenting mouse dendritic cells by reducing the production of the pro-inflammatory cytokines tumour necrosis factor alpha and interleukin-12, and proliferation of antigen-specific CD4(+) T-cells.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2011

Počet záznamů: 1