Počet záznamů: 1
The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors
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SYSNO ASEP 0345026 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors Tvůrce(i) Kubinová, Šárka (UEM-P) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Kozubenko, Nataliya (UEM-P) RID
Vaněček, Václav (UEM-P)
Proks, Vladimír (UMCH-V) RID, ORCID
Price, J. (GB)
Cocks, G. (GB)
Syková, Eva (UEM-P) RIDZdroj.dok. Biomaterials. - : Elsevier - ISSN 0142-9612
Roč. 31, č. 23 (2010), s. 5966-5975Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova cell adhesion ; laminin ; mesenchymal stem cell Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy CEP LC554 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IAA500390902 GA AV ČR - Akademie věd CEZ AV0Z50390703 - UEM-P (2007-2013) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000279719600007 DOI 10.1016/j.biomaterials.2010.04.040 Anotace Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with laminin-derived Ac-CGGASIKVAVS-OH peptide sequences have been developed to construct scaffolds that promote cell adhesion and neural differentiation. The Ac-CGGASIKVAVS-OH peptide was immobilized to the sulfhydryl groups by 2,2'-dithiodipyridine linking reagent via 2-[4-(2-pyridyldisulfanyl)butanamido]ethyl methacrylate. Superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds significantly increased the number of attached mesenchymal stem cells and their growth area on the hydrogel surface in the absence and in the presence of serum in the culture medium. Additionally, the Ac-CGGASIKVAVS-OH peptide supported the attachment, proliferation, differentiation and process spreading of human fetal neural stem cells during the first two weeks of expansion and contributed to the formation of a high percentage of more mature neural cells after four weeks of expansion. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2011
Počet záznamů: 1