Počet záznamů: 1

Probing plant membranes with FM dyes: tracking, dragging or blocking?

SYSNO ASEP	0343962
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Probing plant membranes with FM dyes: tracking, dragging or blocking?
Tvůrce(i)	Jelínková, Adriana (UEB-Q)_{RID, ORCID} Malínská, Kateřina (UEB-Q)_{RID, ORCID} Simon, Sibu (UEB-Q)_ORCID Kleine-Vehn, J. (DE) Pařezová, Markéta (UEB-Q) Pejchar, Přemysl (UEB-Q)_{RID, ORCID} Kubeš, Martin (UEB-Q)_{RID, ORCID} Martinec, Jan (UEB-Q)_{RID, ORCID} Friml, J. (DE) Zažímalová, Eva (UEB-Q)_{RID, ORCID} Petrášek, Jan (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 61, č. 5 (2010), s. 883-892
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	FM 4-64 ; FM 1-43 ; plasma membrane
Vědní obor RIV	ED - Fyziologie
CEP	KJB600380604 GA AV ČR - Akademie věd
	LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000274805600014
DOI	10.1111/j.1365-313X.2009.04102.x
Anotace	Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1