Počet záznamů: 1
Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches
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SYSNO ASEP 0343446 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches Tvůrce(i) Dlouhá, Š. (CZ)
Thielsch, A. (DE)
Kraus, R.H.S. (DE)
Seďa, Jaromír (BC-A) RID
Schwenk, K. (DE)
Petrusek, A. (CZ)Zdroj.dok. Hydrobiologia. - : Springer - ISSN 0018-8158
Roč. 643, č. 1 (2010), s. 107-122Poč.str. 16 s. Akce International Symposium on Cladocera /8./ Datum konání 21.10.2008-25.10.2008 Místo konání Aguascalientes Země MX - Mexiko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Daphnia ; interspecific hybridization ; internal transcribed spacer ; allozyme electrophoresis ; microsatellites Vědní obor RIV DA - Hydrologie a limnologie CEP GA206/04/0190 GA ČR - Grantová agentura ČR IAA600960901 GA AV ČR - Akademie věd CEZ AV0Z60170517 - HBU-Z, BC-A (2005-2011) UT WOS 000276256500014 DOI 10.1007/s10750-010-0128-8 Anotace Daphnia galeata, D. longispina and D. cucullata are closely related species which often produce interspecific hybrids. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. These results warn against its use as a sole identification method in field studies. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2011
Počet záznamů: 1