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Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro
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SYSNO ASEP 0343392 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro Tvůrce(i) Polanská, M. (CZ)
Hulejová, H. (CZ)
Petrtýl, M. (CZ)
Bastl, Zdeněk (UFCH-W) RID, ORCID
Spirovová, Ilona (UFCH-W) RID
Kruliš, Zdeněk (UMCH-V) RID, ORCID
Horák, Zdeněk (UMCH-V)
Veigl, D. (CZ)
Šenolt, L. (CZ)Zdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 59, č. 2 (2010), s. 247-253Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova osteochondral defects ; cycloolefin copolymer ; chondrocytes ; biocompatibility Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP GA106/06/0761 GA ČR - Grantová agentura ČR CEZ AV0Z40400503 - UFCH-W (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000278016200012 Anotace Materials on the basis of cycloolefin copolymers (COC) are suitable for subchondral defect repairs. The objective of this study was to evaluate the influence of surface modification of COC and COC/LLDPE blends on the viability and gene expression of chondrocytes. Human chondrocytes were incubated on the surface of the studied materials. Half of the materials were plasmatically modified with a subsequent type II collagen application. The gene expression of matrix metalloproteinases (MMP-1,-3,-13), pro-inflammatory cytokines (IL-1, TNF-alpha) and apoptotic molecules (BAX, Bcl-2) was evaluated using quantitative Taq-Man PCR after 48 h incubation. Chondrocyte viability was evaluated by the MTT test after 2, 4 and 8 days of incubation. The synthesis of MMPs was measured by ELISA assay in cell culture medium after 48 h of incubation. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2011
Počet záznamů: 1