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Lipopolythiourea/DNA interaction: A biophysical study
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SYSNO ASEP 0342982 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lipopolythiourea/DNA interaction: A biophysical study Tvůrce(i) Kral, Teresa (UFCH-W) RID, ORCID
Leblond, J. (FR)
Hof, Martin (UFCH-W) RID, ORCID
Scherman, D. (FR)
Hersovici, J. (FR)
Mignet, N. (FR)Zdroj.dok. Biophysical Chemistry. - : Elsevier - ISSN 0301-4622
Roč. 148, 1-3 (2010), s. 68-73Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova lipopolythiourea ; FCS ; plasmid DNA compaction ; 31P NMR Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP IAA400400621 GA AV ČR - Akademie věd LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000277743900009 DOI 10.1016/j.bpc.2010.02.011 Anotace Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2011
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