Počet záznamů: 1

Probing for primary functions of prohibitin in Trypanosoma brucei

SYSNO ASEP	0342888
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Probing for primary functions of prohibitin in Trypanosoma brucei
Tvůrce(i)	Týč, Jiří (BC-A)_{ORCID, RID} Faktorová, Drahomíra (BC-A)_{RID, ORCID} Kriegová, Eva (BC-A)_SAI Jirků, Milan (BC-A)_RID Vávrová, Zuzana (BC-A) Maslov, D. A. (US) Lukeš, Julius (BC-A)_{RID, ORCID}
Zdroj.dok.	International Journal for Parasitology. - : Elsevier - ISSN 0020-7519 Roč. 40, č. 1 (2010), s. 73-83
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	AU - Austrálie
Klíč. slova	prohibitin ; mitochondrion ; morphology ; mitochondrial translation
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA204/09/1667 GA ČR - Grantová agentura ČR
	IAA500960705 GA AV ČR - Akademie věd
	GP204/06/P423 GA ČR - Grantová agentura ČR
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000274104300008
DOI	10.1016/j.ijpara.2009.07.008
Anotace	We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2011

Počet záznamů: 1