Počet záznamů: 1
Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes
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SYSNO ASEP 0341720 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Tvůrce(i) Filippov, Sergey K. (UMCH-V) RID, ORCID, SAI
Koňák, Čestmír (UMCH-V) RID
Kopečková, P. (US)
Starovoytova, Larisa (UMCH-V) RID
Špírková, Milena (UMCH-V) RID, ORCID
Štěpánek, Petr (UMCH-V) RID, ORCIDZdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 26, č. 7 (2010), s. 4999-5006Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DNA ; polyplex ; cholesterol Vědní obor RIV BO - Biofyzika CEP GA202/09/2078 GA ČR - Grantová agentura ČR CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000275995100068 DOI 10.1021/la9036716 Anotace Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2011
Počet záznamů: 1