Počet záznamů: 1
Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice
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SYSNO ASEP 0340102 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice Tvůrce(i) Matoušek, Jaroslav (BC-A) RID, ORCID
Matoušek, Josef (UZFG-Y) RIDCelkový počet autorů 2 Zdroj.dok. Recent Patents on DNA & Gene Sequences - ISSN 1872-2156
Roč. 4, č. 1 (2010), s. 29-39Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova antiproliferative cytotoxic ; effect human ; plant nuclease Vědní obor RIV EB - Genetika a molekulární biologie CEP GA521/06/1149 GA ČR - Grantová agentura ČR GA521/09/1214 GA ČR - Grantová agentura ČR CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) Anotace Recombinant plant nucleases of tomato (TBN1) and hop (HBN1) (submitted to patenting under no. PV 2008-384;Z7585) were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). However, the antitumor effect of the first nuclease tested, the mung bean nuclease (PhA), on ML2 human tumor cell line was neither effective, even when significant antitumor activity was detected on human melanoma tumors in vivo. Recombinant TBN1 and HBN1 were as effective against human melanoma tumors as previously studied pine pollen nuclease and PhA and their effects were reached at about ten times lower concentrations compared to the use of bovine seminal RNase (BS-RNase). Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
Počet záznamů: 1