Počet záznamů: 1
Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter
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SYSNO ASEP 0337485 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter Překlad názvu Stimulace kvetení a větvení v chryzantémách exprimujících gen ipt pod kontrolou 0.821 kb fragmentu promotoru genu LEACO1 Tvůrce(i) Khodakovskaya, M. (RU)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Li, A. (US)
Li, Y. (US)
McAvoy, R. (US)Zdroj.dok. Plant Cell Reports. - : Springer - ISSN 0721-7714
Roč. 28, č. 9 (2009), s. 1351-1362Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova ipt Gene ; Cytokinins ; Transgenic chrysanthemum Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000269208100006 DOI 10.1007/s00299-009-0735-x Anotace The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium ‘Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
Počet záznamů: 1