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Functional p53 in cells contributes to the anticancer effect of the cyclin-dependent kinase inhibitor roscovitine
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SYSNO ASEP 0335831 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Functional p53 in cells contributes to the anticancer effect of the cyclin-dependent kinase inhibitor roscovitine Překlad názvu Funkční p53 v buňkách přispívá k protinádorovému účinku roskovitinu Tvůrce(i) Paprskářová, Martina (UEB-Q)
Kryštof, Vladimír (UEB-Q) RID, ORCID
Jorda, Radek (UEB-Q) ORCID, RID
Džubák, P. (CZ)
Hajdúch, M. (CZ)
Wesierska-Gadek, J. (AT)
Strnad, Miroslav (UEB-Q) RID, ORCIDZdroj.dok. Journal of Cellular Biochemistry. - : Wiley - ISSN 0730-2312
Roč. 107, č. 3 (2009), s. 428-437Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova APOPTOSIS ; CYCLIN-DEPENDENT KINASE ; OLOMOUCINE II ; p53 Vědní obor RIV EB - Genetika a molekulární biologie CEP GA204/08/0511 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000266872300007 DOI 10.1002/jcb.22139 Anotace To define the role of p53 in the anticancer effect of selective CDK inhibitors, two related compounds roscovitine and olomoucine II were studied. Roscovitine differs functionally from its congener olomoucine II only in the selectivity towards transcriptional CDK9. Action of both compounds on proliferation, cell cycle progression and apoptosis was examined in RPMI-8226 cells expressing the temperature-sensitive mutant of p53 and in MCF-7 cells with wild-type p53. Both compounds blocked proliferation, decreased phosphorylation of RNA polymerase II, downregulated anti-apoptotic protein Mcl-1 in both cell lines in a dose-dependent manner, and also activated p53 in MCF-7 cells. Moreover, we showed that the anticancer efficiency of CDK inhibitors was enhanced by active p53 in RPMI-8226 cells kept at permissive temperature, where downregulation of Mcl-1, fragmentation of PARP-1 and increased caspase-3 activity was detected with lower doses of the compounds. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
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