Počet záznamů: 1
DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model
- 1.
SYSNO ASEP 0335722 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model Tvůrce(i) Furugaki, K. (FR)
Pokorná, Kateřina (UMG-J)
le Pogam, C. (FR)
Aoki, M. (FR)
Reboul, M. (FR)
Bajzik, V. (FR)
Krief, P. (FR)
Janin, A. (FR)
Noguera, M.-E. (FR)
West, R. (GB)
Charron, D. (FR)
Chomienne, C. (FR)
Pla, M. (FR)
Moins-Teisserenc, H. (FR)
Padua, R.A. (FR)Celkový počet autorů 15 Zdroj.dok. Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 115, č. 3 (2010), s. 653-656Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova all-trans retinoic acid ; DNA vaccination ; protective immunity Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000273820600027 DOI 10.1182/blood-2007-08-109009 Anotace DNA vaccination and all-trans retinoic acid (ATRA) bring survival advantage in a mouse model of acute promyelocytic leukemia (APL). Depletion of CD4+ or CD8+ cells abolished this effect. CD4+ depletions of long-term survivors resulted in relapse and death within 3 months, thus demonstrating need of both cell subsets for generation of DNA-driven antileukemic immune responses and underscoring a crucial role of CD4+ cells in maintenance of durable remissions. Degranulation and cytotoxic carboxyfluorescein diacetate succinimidyl ester-based assays showed MHC-restricted APL-specific T cell-mediated immune responses. Sorted APL-specific CD8+CD107a+ T cells showed increased antileukemic activity. Effectors from ATRA + DNA-treated mice were shown to secrete IFN-g when stimulated with APL cells or peptides from the PL-RARa vaccine-derived sequences as shown by ELISpot assays. DNA vaccination with ATRA thus confers effective boosting of IFN-g-producing and cytotoxic T cells in leukemic mice. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1