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Profiling ABA metabolites in Nicotiana tabacum L. leaves by ultra-performance liquid chromatography–electrospray tandem mass spectrometry
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SYSNO ASEP 0332937 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Profiling ABA metabolites in Nicotiana tabacum L. leaves by ultra-performance liquid chromatography–electrospray tandem mass spectrometry Překlad názvu Analýza ABA metabolitů v listech Nicotiana tabacum L. pomocí UPLC-ESI-MS/MS Tvůrce(i) Turečková, Veronika (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCIDZdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 80, č. 1 (2009), s. 390-399Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Abscisic acid ; Ultra-performance liquid chromatography (UPLC) ; Tandem mass spectrometry (MS/MS) Vědní obor RIV EC - Imunologie CEP KAN200380801 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000271055700057 DOI 10.1016/j.talanta.2009.06.027 Anotace We have developed a simple method for extracting and purifying (+)-abscisic acid (ABA) and eight ABA metabolites – before analysis by a novel technique for these substances, ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The procedure includes addition of deuterium-labelled standards, extraction with methanol-water-acetic acid (10:89:1, v/v), simple purification by Oasis® HLB cartridges, rapid chromatographic separation by UPLC, and sensitive, accurate quantification by MS/MS in multiple reaction monitoring modes. The detection limits of the technique ranged between 0.1 - 1 pmoL for ABAGE and ABA acids in negative ion mode, and 0.01 – 0.50 pmoL for ABAGE, ABAaldehyde, ABAalcohol and the methylated acids in positive ion mode. We isolated ABA and its metabolites from control and water-stressed tobacco leaf tissues. Only ABA, PA, DPA, neoPA, and ABAGE were detected in the samples. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
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