Počet záznamů: 1
Effect of antipsychotic drug perphenazine on fast sodium current and transient outward potassium current in rat ventricular myocytes
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SYSNO ASEP 0330554 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of antipsychotic drug perphenazine on fast sodium current and transient outward potassium current in rat ventricular myocytes Překlad názvu Vliv antipsychotika perfenazinu na rychlý sodíkový proud a přechodný draslíkový proud u komorových srdečních buněk potkana Tvůrce(i) Bébarová, M. (CZ)
Matejovič, P. (CZ)
Pásek, Michal (UT-L) RID, ORCID
Jansová, D. (CZ)
Šimurdová, M. (CZ)
Nováková, M. (CZ)
Šimurda, J. (CZ)Zdroj.dok. Naunyn-Schmiedeberg's Archives of Pharmacology. - : Springer - ISSN 0028-1298
Roč. 380, č. 2 (2009), s. 125-133Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova perphenazine ; antipsychotic drug ; sodium current ; transient outward current ; rat ventricular myocytes Vědní obor RIV ED - Fyziologie CEZ AV0Z20760514 - UT-L (2005-2011) UT WOS 000266926900004 DOI 10.1007/s00210-009-0420-1 Anotace The effect of antipsychotic drug perphenazine (0.1–100 ?M) on fast sodium current INa and on transient outward potassium current Ito was analysed in enzymatically isolated rat right ventricular myocytes by the whole-cell patch-clamp technique at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50=1.24±0.10 ?M) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50=38.2±3.5 ?M, evaluated from changes of the time integral). The fast time constant of Ito inactivation was significantly decreased in a concentration-dependent manner (IC50=30.0±6.6 ?M). Both blocks were use and frequency dependent at 3.3 Hz. We conclude that perphenazine cause concentration-,use-,and frequency-dependent block of INa and Ito. Computer simulations suggest that perphenazine interacts preferentially with INa channels in inactivated states and with Ito channels in both open and open-inactivated s tates. Pracoviště Ústav termomechaniky Kontakt Marie Kajprová, kajprova@it.cas.cz, Tel.: 266 053 154 ; Jana Lahovská, jaja@it.cas.cz, Tel.: 266 053 823 Rok sběru 2010
Počet záznamů: 1