Počet záznamů: 1  

Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin

  1. 1.
    SYSNO ASEP0330518
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevOligomerization is involved in pore formation by Bordetella adenylate cyclase toxin
    Tvůrce(i) Vojtová, Jana (MBU-M)
    Basler, Marek (MBU-M)
    Osička, Radim (MBU-M) RID
    Knapp, O. (DE)
    Maier, E. (DE)
    Černý, J. (CZ)
    Benada, Oldřich (MBU-M)
    Benz, R. (DE)
    Šebo, Peter (MBU-M) RID
    Zdroj.dok.FASEB Journal - ISSN 0892-6638
    Roč. 23, - (2009), s. 2831-2843
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovablue native electrophoresis ; planar lipid bilayer membranes ; pore-forming activity
    Vědní obor RIVEE - Mikrobiologie, virologie
    CEPIAA500200914 GA AV ČR - Akademie věd
    1M0506 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    CEZAV0Z50200510 - MBU-M (2005-2011)
    UT WOS000270241000006
    DOI10.1096/fj.09-131250
    AnotaceThe Bordetella adenylate cyclase-hemolysin (CyaA, ACT, or AC-Hly) is a multifunctional toxin. CyaA delivers into host cells an adenylate cyclase enzyme (AC) and permeabilizes cell membrane by forming small cation selective pores. Indirect evidence suggested that these two activities were accomplished by different membrane-inserted CyaA conformers, one acting as an AC-delivering monomer, the other as an uncharacterized pore-forming oligomer. Here, CyaA oligomers were revealed in sheep erythrocyte membranes by immunogold labeling and directly demonstrated by pull-down of membrane-inserted CyaA together with biotinylated CyaA-AC- toxoid. Membrane oligomers of CyaA could also be resolved by non-denaturing electrophoresis of mild detergent extracts of erythrocytes
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2010