Počet záznamů: 1

Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin

SYSNO ASEP	0330518
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin
Tvůrce(i)	Vojtová, Jana (MBU-M)_{RID, ORCID} Basler, Marek (MBU-M) Osička, Radim (MBU-M)_{RID, ORCID} Knapp, O. (DE) Maier, E. (DE) Černý, J. (CZ) Benada, Oldřich (MBU-M)_{ORCID, RID} Benz, R. (DE) Šebo, Peter (MBU-M)_{RID, ORCID}
Zdroj.dok.	FASEB Journal. - : Wiley - ISSN 0892-6638 Roč. 23, - (2009), s. 2831-2843
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	blue native electrophoresis ; planar lipid bilayer membranes ; pore-forming activity
Vědní obor RIV	EE - Mikrobiologie, virologie
CEP	IAA500200914 GA AV ČR - Akademie věd
	1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000270241000006
DOI	10.1096/fj.09-131250
Anotace	The Bordetella adenylate cyclase-hemolysin (CyaA, ACT, or AC-Hly) is a multifunctional toxin. CyaA delivers into host cells an adenylate cyclase enzyme (AC) and permeabilizes cell membrane by forming small cation selective pores. Indirect evidence suggested that these two activities were accomplished by different membrane-inserted CyaA conformers, one acting as an AC-delivering monomer, the other as an uncharacterized pore-forming oligomer. Here, CyaA oligomers were revealed in sheep erythrocyte membranes by immunogold labeling and directly demonstrated by pull-down of membrane-inserted CyaA together with biotinylated CyaA-AC- toxoid. Membrane oligomers of CyaA could also be resolved by non-denaturing electrophoresis of mild detergent extracts of erythrocytes
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2010

Počet záznamů: 1