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AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes
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SYSNO ASEP 0329884 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes Tvůrce(i) Kalous, Jaroslav (UZFG-Y) RID, ORCID
Kubelka, Michal (UZFG-Y) RID, ORCID
Šolc, Petr (UZFG-Y) RID, ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Motlík, Jan (UZFG-Y) RID, ORCIDZdroj.dok. Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 138, - (2009), s. 645-654Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova protein kinase ; porcine oocyte ; oocyte maturation Vědní obor RIV CE - Biochemie CEP GA204/06/1297 GA ČR - Grantová agentura ČR GA523/03/0857 GA ČR - Grantová agentura ČR GA524/07/1087 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000270806100004 DOI https://doi.org/10.1530/REP-08-0461 Anotace The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
Počet záznamů: 1