Počet záznamů: 1

AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes

SYSNO ASEP	0329884
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes
Tvůrce(i)	Kalous, Jaroslav (UZFG-Y)_{RID, ORCID} Kubelka, Michal (UZFG-Y)_{RID, ORCID} Šolc, Petr (UZFG-Y)_{RID, ORCID} Šušor, Andrej (UZFG-Y)_{RID, ORCID} Motlík, Jan (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Reproduction. - : BioScientifica - ISSN 1470-1626 Roč. 138, - (2009), s. 645-654
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	protein kinase ; porcine oocyte ; oocyte maturation
Vědní obor RIV	CE - Biochemie
CEP	GA204/06/1297 GA ČR - Grantová agentura ČR
	GA523/03/0857 GA ČR - Grantová agentura ČR
	GA524/07/1087 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000270806100004
DOI	10.1530/REP-08-0461
Anotace	The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2010

Počet záznamů: 1