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Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants
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SYSNO ASEP 0328462 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants Překlad názvu Regulace fosfoenolpyruvátkarboxylasy v rostlinách tabáku infikovaných virem PVYNTN Tvůrce(i) Müller, Karel (UEB-Q) RID, ORCID
Doubnerová, V. (CZ)
Synková, Helena (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Ryšlavá, H. (CZ)Zdroj.dok. Biological Chemistry. - : Walter de Gruyter - ISSN 1431-6730
Roč. 390, č. 3 (2009), s. 245-251Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova biotic stress ; Nicotiana tabacum ; phosphoenolpyruvate carboxylase (PEPC) Vědní obor RIV ED - Fyziologie CEP GA206/03/0310 GA ČR - Grantová agentura ČR 1M0505 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000264921500005 DOI 10.1515/BC.2009.029 Anotace The effect of viral infection on regulation of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves was studied. The PEPC activity was 3 times higher in infected plant leaves as compared to healthy plants. The reason of an increase of PEPC activity in consequence of PVYNTN infection was studied. The amount of PEPC determined by Western blot analysis or qRT PCR of PEPC mRNA did not differ in the control and PVYNTN infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation. Immunochemical detection of phosphoproteins by Western blot analysis showed more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plants response to pathogen attack. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2010
Počet záznamů: 1