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Gene expression during minor genome activation in preimplantation bovine development
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SYSNO ASEP 0328317 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gene expression during minor genome activation in preimplantation bovine development Tvůrce(i) Kaňka, Jiří (UZFG-Y) RID, ORCID
Kepková, Kateřina (UZFG-Y)
Němcová, Lucie (UZFG-Y) RIDZdroj.dok. Theriogenelogy. - : Elsevier - ISSN 0093-691X
Roč. 72, - (2009), s. 572-583Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova developmental biology ; embryo ; gene expression ; real-time RT-PCR Vědní obor RIV GI - Šlechtění a plemenářství hospodářských zvířat CEP GA523/06/1226 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000268901700019 DOI 10.1016/j.theriogenology.2009.04.014 Anotace The main goal of this study was to identify mRNA transcripts, whose content increases during bovine minor embryonic genome activation. We compared the gene expression profile of the bovine 4-cell stage embryo and MII oocyte using the technique of suppression subtractive hybridization. Differentially expressed amplicons were subcloned and 60 of them were sequenced. The resulting DNA sequences were compared with GenBank databases using Blast search. The expression of five differentially expressed genes with an apparent function in cell cycle progression, chromatin remodeling, and splicing or translation initiation was further characterized by a real-time RT-PCR. Splicing factor, arginine/serine-rich 3 (SRFS3) represents the first described gene with an important function in pre-implantation development, which is also expressed during bovine minor genome activation and it is alpha-amanitin sensitive during this period. All described genes can play an important role in the pre-implantation development of bovine embryos. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
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