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Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli
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SYSNO ASEP 0328089 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli Překlad názvu Produkce cyklodextrin glukanotransferasy buňkami alkalifilních bacilů Tvůrce(i) Atanasova, N. (BG)
Kitayska, T. (BG)
Yankov, D. (BG)
Šafaříková, Miroslava (UEK-B) RID, SAI
Tonkova, A. (BG)Zdroj.dok. Biochemical Engineering Journal. - : Elsevier - ISSN 1369-703X
Roč. 46, - (2009), s. 278-285Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova alkaliphilic bacilli ; immobilization Vědní obor RIV CE - Biochemie CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000274797800006 Anotace Cells of Bacillus pseudalcaliphilus 20RF and Bacillus pseudalcaliphilus 8SB isolated from Bulgarian habitats, producers of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19), were immobilized by three different techniques: on two types of polysulphone membranes; entrapped in agar-gel beads containing magnetite and by nano-particles of silanized magnetite covalently bound on the cell surface. The biocatalysts obtained demonstrated the opportunity for a significantly enhanced CGTase production compared to free cells for a long period of time (10 days semicontinuous cultivation) without impact on their mechanical stability. The cell membrane-biocatalysts exhibited the highest enzyme aktivity. Membrane biocatalysts were applied for a direct cyclodextrin production. The results obtained demonstrated the possibility of starch conversion into cyclodextrins by immobilized cells. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2010
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