Počet záznamů: 1
Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes
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SYSNO ASEP 0323300 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes Překlad názvu Vliv vybraných endokrinních disruptorů na meiotické zrání, expanzi kumulu, syntézu hyaluronové kyseliny a progesteronu oocyt-kumulárními komplexy prasat. Tvůrce(i) Mlynarčíková, A. (SK)
Nagyová, Eva (UZFG-Y) RID, ORCID
Ficková, M. (SK)
Scsuková, S. (SK)Zdroj.dok. Toxicology in Vitro. - : Elsevier - ISSN 0887-2333
Roč. 23, - (2009), s. 371-377Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova oocyte-cumulus complex ; meiotic maturation ; cumulus expansion Vědní obor RIV EB - Genetika a molekulární biologie CEP GA305/05/0960 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000264510200002 DOI 10.1016/j.tiv.2008.12.017 Anotace Before ovulation, cumulus cells synthesize a large amount of hyaluronan (HA) that is organized in an extracellular matrix.This process is called cumulus expansion. We assessed effects of endocrine disruptors (bisphenol A, BPA, 4-chloro-3-methyl phenol, CMP, di(2-ethylhexyl) phthalate, DEHP, and benzyl butyl phthalate, BBP) on FSH-induced meiotic maturation of porcine oocyte-cumulus complexes (OCC) cultured in vitro. BPA and CMP, significantly affected meiotic maturation of OCC. The number of oocytes that underwent GVBD; 78.7% and 72.4% as well as the rate of oocytes that reached M II; 50% and 53.6%, were decreased compared to control: GVBD-89.6% ; M II- 81.5%. FSH-stimulated expansion of cumulus was altered by the highest concentration of BPA and CMP (70% and 64% vs. 80.3% in control). Although BPA did not alter FSH-stimulated HA synthesis by cumulus cells, its incorporation within the complex was reduced to a half of control value. Progesterone production by OCC was significantly changed in the presence of BPA or DEHP. Our results provide valuable information that oocyte development was adversely affected during in vitro culture with endocrine disruptors. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2009
Počet záznamů: 1