Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae

Vrbová, Iva; Neven, L. G.; Bárcenas, N. M.; Gund, N. A.; Dalíková, Martina; Marec, František

doi:https://dx.doi.org/10.1111/j.1439-0418.2008.01352.x

Počet záznamů: 1

Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae

1.

SYSNO ASEP	0322993
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae
Překlad názvu	Rychlé stanovení pohlaví u vajíček a larev obaleče jablečného Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae)
Tvůrce(i)	Vrbová, Iva (BC-A)_{RID, ORCID} Neven, L. G. (US) Bárcenas, N. M. (US) Gund, N. A. (DE) Dalíková, Martina (BC-A)_{RID, ORCID} Marec, František (BC-A)_{RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Journal of Applied Entomology. - : Wiley - ISSN 0931-2048 Roč. 133, č. 4 (2009), s. 249-261
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	Lepidoptera ; molecular markers ; period gene
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA206/06/1860 GA ČR - Grantová agentura ČR
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000265035600003
DOI	10.1111/j.1439-0418.2008.01352.x
Anotace	Two different methods were tested to identify the sex of the early developmental stages of the codling moth with a WZ/ZZ (female/male) sex chromosome system. First, it was shown that the sex of larvae can be easily determined by the presence or absence of sex chromatin, which is formed by the W chromosome in interphase nuclei of females. Secondly, a new sexing technique was developed based on the molecular marker CpW2-EcoRI of the W chromosome. For this technique, a fragment of a codling moth gene, period (Cpper), was isolated and sequenced. Results of southern hybridization suggested that the Cpper gene is located on the Z chromosome. Then a multiplex PCR assay was developed, which co-amplified the CpW2-EcoRI sequence to identify the W chromosome and the Z-linked Cpper sequence, which served as a positive control of accurate processing of tested samples. The multiplex PCR provides an easy and rapid identification of the sex of embryos and early larval instars of the codling moth.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2009

Počet záznamů: 1