Počet záznamů: 1
Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae
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SYSNO ASEP 0322993 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae Překlad názvu Rychlé stanovení pohlaví u vajíček a larev obaleče jablečného Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) Tvůrce(i) Vrbová, Iva (BC-A) RID, ORCID
Neven, L. G. (US)
Bárcenas, N. M. (US)
Gund, N. A. (DE)
Dalíková, Martina (BC-A) RID, ORCID
Marec, František (BC-A) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Journal of Applied Entomology. - : Wiley - ISSN 0931-2048
Roč. 133, č. 4 (2009), s. 249-261Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Lepidoptera ; molecular markers ; period gene Vědní obor RIV EB - Genetika a molekulární biologie CEP GA206/06/1860 GA ČR - Grantová agentura ČR CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000265035600003 DOI https://doi.org/10.1111/j.1439-0418.2008.01352.x Anotace Two different methods were tested to identify the sex of the early developmental stages of the codling moth with a WZ/ZZ (female/male) sex chromosome system. First, it was shown that the sex of larvae can be easily determined by the presence or absence of sex chromatin, which is formed by the W chromosome in interphase nuclei of females. Secondly, a new sexing technique was developed based on the molecular marker CpW2-EcoRI of the W chromosome. For this technique, a fragment of a codling moth gene, period (Cpper), was isolated and sequenced. Results of southern hybridization suggested that the Cpper gene is located on the Z chromosome. Then a multiplex PCR assay was developed, which co-amplified the CpW2-EcoRI sequence to identify the W chromosome and the Z-linked Cpper sequence, which served as a positive control of accurate processing of tested samples. The multiplex PCR provides an easy and rapid identification of the sex of embryos and early larval instars of the codling moth. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2009
Počet záznamů: 1