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Short-term variation in extracellular phosphatase activity: Possible limitations for diagnosis of nutrient status in particular algal populations
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SYSNO ASEP 0321203 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Short-term variation in extracellular phosphatase activity: Possible limitations for diagnosis of nutrient status in particular algal populations Překlad názvu Krátkodobá variabilita extracelulární fosfatázové aktivity: Možná omezení pro diagnózu stavu živin v jednotlivých řasových populacích Tvůrce(i) Štrojsová, A. (CZ)
Vrba, Jaroslav (BC-A) RID, ORCIDZdroj.dok. Aquatic Ecology. - : Springer - ISSN 1386-2588
Roč. 43, č. 1 (2009), s. 19-25Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ELF97 phosphate ; ectoenzyme activity ; diurnal ; Ankyra ancora ; Chlorogonium fusiforme ; Monoraphidium dybowski Vědní obor RIV EH - Ekologie - společenstva CEP IAA6017202 GA AV ČR - Akademie věd CEZ AV0Z60170517 - HBU-Z, BC-A (2005-2011) UT WOS 000263159200002 DOI 10.1007/s10452-007-9154-7 Anotace Species-specific extracellular phosphatase activity (PA) was studied in natural phytoplankton with an emphasis on short-term variation in PA and its consequences for the diagnosis of phosphorus (P) deficiency. PA was directly detected by epifluorescence microscopy using fluorescently labelled enzyme activity and quantified using image analysis. High short-term variation was found both in the total PA of the samples and specific PA of Ankyra ancora, Monoraphidium dybowski, and Chlorogonium fusiforme. The specific PA of these species varied between 0 and 157 fmol cell–1 h–1. The PA of Chlorogonium fusiforme showed a significant relationship with the time of day. Populations of the certain species were present that differed in physiological status and activity due to diurnal or random changes caused by mixing in lakes. Thus, species-specific extracellular PA should be interpreted carefully as an indicator of P deficiency in freshwater phytoplankton if based on limited number of samplings. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2009
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