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A new (14th) member of Borrelia burgdorferi sensu lato complex
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SYSNO ASEP 0320267 Druh ASEP A - Abstrakt Zařazení RIV Není vybrán druh dokumentu Název A new (14th) member of Borrelia burgdorferi sensu lato complex Překlad názvu Nový (14.) člen komplexu Borrelia burgdorferi sensu lato Tvůrce(i) Oliver, J. (US)
Rudenko, Natalia (BC-A) RID, ORCID
Golovchenko, Maryna (BC-A) RID, ORCID
Grubhoffer, Libor (BC-A) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. Clinical Microbiology and Infection - ISSN 1198-743X
Roč. 14, S7 (2008), S110-S110Poč.str. 1 s. Akce ECCMID /18./ Datum konání 19.04.2008-22.04.2008 Místo konání Barcelona Země ES - Španělsko Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CA - Kanada Klíč. slova Borrelia burgdorferi s.l. complex ; new member ; multilocus sequence analysis Vědní obor RIV EC - Imunologie CEP GA524/06/1479 GA ČR - Grantová agentura ČR LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Anotace Recently we studied 118 Borrelia isolates, cultured from samples collected from a variety of birds, rodents and ticks in 9 different localities of the southern region of the Unites States. A group of 16 isolates with unusual characteristics was found. The new methodology of multilocus sequence analysis (MLSA) that involved the study of rrf-rrl intergenic spacer region, rrs (16S RNA) gene, flagellin gene, ospA gene and p66 gene was used to clarify the taxonomic status of this new highly homogeneous group. All 13 recognized borrelia species were used as controls. The association of this species with Lyme disease group was confirmed by multiple results of MLSA. Unique RFLP pattern was discovered in the rrf-rrl intergenic spacer and flagellin gene. Unique signature nucleotides were allocated in the 16S rRNA. Phylogenetic analysis based on concatenated sequences of 5 loci showed all 16 isolates clustered together but separately from the other species of B. burgdorferi s.l. complex. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2009
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